| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 291 | Thermosipho melanesiensis | TGTTGCATGAAGGACCTGGA | ACCGCTCCGTCTACTGTCA | 59.23 | 60.30 | 211 | 99 |
100.00%
|
100.00%
|
| Primer 292 | Thermosipho melanesiensis | TCGCATCATCCATAGGGCA | AGTCGTGTTGTCCGGATCA | 58.55 | 58.65 | 266 | 99 |
100.00%
|
100.00%
|
| Primer 293 | Thermosipho melanesiensis | CGTTATAGCAAGGATCGCGTC | GGAGCACCTCCTAAAGCTTCA | 59.29 | 59.72 | 234 | 99 |
100.00%
|
100.00%
|
| Primer 294 | Thermosipho melanesiensis | TCGCGTCAATAATCAAGCACA | GGAGCACCTCCTAAAGCTTCA | 58.66 | 59.72 | 220 | 99 |
100.00%
|
100.00%
|
| Primer 295 | Thermosipho melanesiensis | CGCGTCAATAATCAAGCACAG | GGAGCACCTCCTAAAGCTTCA | 58.30 | 59.72 | 219 | 99 |
100.00%
|
100.00%
|
| Primer 296 | Thermosipho melanesiensis | GTTATAGCAAGGATCGCGTCA | GGAGCACCTCCTAAAGCTTCA | 58.26 | 59.72 | 233 | 99 |
100.00%
|
100.00%
|
| Primer 297 | Thermosipho melanesiensis | TGCGAAGGTGGTACGTGA | CTCCAAACAAAGGCACCCC | 58.55 | 58.95 | 266 | 99 |
100.00%
|
100.00%
|
| Primer 298 | Thermosipho melanesiensis | ACTCACGGTGTAGGAGCTTT | ACCCACACTACCGCAAACA | 58.66 | 59.47 | 293 | 98 |
100.00%
|
100.00%
|
| Primer 299 | Thermosipho melanesiensis | TGGCACACAGTATACACAGGA | TGCCGTATGCTCCTGCTTT | 58.75 | 59.70 | 157 | 98 |
100.00%
|
100.00%
|
| Primer 300 | Thermosipho melanesiensis | GCTGTTGCTGGTGCGTTT | AGTTGCGTAGGCGTCTGAA | 59.59 | 59.34 | 285 | 98 |
100.00%
|
100.00%
|